Meso Scale Discovery Assay: How to Pick Suitable Kit Class for Your Biomarker Testing?

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What is Meso Scale Discovery?

Meso Scale Discovery (MSD) is a leading player in the development and manufacture of innovative instruments and assays for measuring molecules and analytes in biological samples. The product line consists of a broad spectrum of biological assay kits ranging from msd cytokine and profiling biomarkers to kits for cell signaling pathways.

For biomarker testing, MSD has specialized msdelisa capable of rapid and convenient estimation of targets in small-volume samples. From personalized msd assay to completely validated assay options, MSD has an ideal assay product for all the demands of an immunoassay. For making it simple for researchers, MSD offers five classes of assay kits for biomarker testing. Each assay class of assay kits presents unique benefits to the user, and therefore the type of kit class depends on the intended use of the product. So let us study each of these kit classes to understand how to pick a suitable assay class for biomarker testing.

R-Plex Assay Platform

R-Plex assays are matched antibody sets that can be adapted into both single and multiplex assays. Each assay kit contains a capture antibody (biotinylated), labeled detection antibody (SULFO-TAG), and a calibrator. The assay kit is tested with serum and plasma samples and comes with MSD Multi-Array capabilities. Moreover, R-Plex assays have a wide range of emerging biomarkers.

U-Plex Assay Platform

U-Plex assay platforms are flexible, rapid, and customized msd mesoscale. It has over 200 assay options providing a level of flexibility never seen before. These assays can be used as standalone kits or combined with other assays for creating customized multiplex combinations. The assays are compatible with EDTA plasma, serum, and cell culture supernatants.

T-Plex assay kits

T-Plex assays are single and multiplex assay formats capable of rapid and timely measurement of biomarker levels in single and small volume samples. These kits are grouped into ultra-sensitive, tissue culture, serum/plasma, whole-cell lysate, and base kits. Secreted biomarker T-Plex assays are tested with plasma, serum, and cell culture supernatants, whereas intracellular T-Plex assays are examined with cell lysates.

V-Plex assay kits

V-Plex assay kits are validated immunoassays with demonstrated delivery of reliable and reproducible assay results. V-Plex assays offer high sensitivity, consistency, and exceptional dynamic range. They come in both pre-configured multiplex panels and individual analyte kits. V-Plex assays are tested with plasma, serum, urine, cell culture supernatants, and CSF.

S-Plex assay kits

S-Plex assays are ultra-sensitive assay kits. They can dramatically reduce the lower limit of detection capabilities by 10- to 1000-fold, making detection in low femtogram/ml levels a routine analysis. Such improved sensitivity reduces the amount of sample volume and critical reagents required for analysis. S-Plex kits are tested for EDTA plasma, serum, heparin plasma, citrate plasma, and cell culture supernatants.


As a plethora of assay kits are available for biomarker assay testing, choosing the right type of assay class is difficult. Therefore, knowing the intended use of an assay protocol increases the chances of selecting a suitable kit class for biomarker testing. For biomarker testing, MSD has specialized msdelisa capable of rapid and convenient estimation of targets in small-volume samples